Visualisation

The recovery and visualisation of the plate using thin-layer chromatography (TLC) are the final steps in the TLC/HPLC protocol. They provide the results of the analysis or serve as a starting point for further analysis. In some cases, visual inspection of the results under visible light is sufficient. However, for most substances to be detected, UV illumination of the TLC/VETLC plate is required. UV detection methods are among the most sensitive detection methods in thin-layer chromatography. At 366 nm, fluorescent substances glow against a dark background. This method becomes even more sensitive as the light intensity increases. To enhance the contrast, it is important that the visible light is filtered. At 254 nm, when using TLC/HPTLC plates containing a fluorescent indicator, the substances appear as dark spots against a bright fluorescent background. UV lamps with a wavelength of 302 nm offer the advantage of combining high sensitivity with a reduced risk of damage to even the most fragile samples. Light intensity and visible light filtration are less critical for this form of detection. We offer models that can be viewed under UV light either in a dark room or outside one.

254/365 nm UV lamp (6 W)

The BS131.500 UV lamp is designed for UV observation of TLC plates in the 254 and 366 nm range. Easy to use and versatile, it can be used handheld or on a stand. Advantages

A protective filter blocks visible light.

Features

The 254 nm (6 W) and 365 nm (6 W) UV lamps can be switched on separately.
Stand included.
Dimensions: 440 x 75 x 51 mm.

Blue Box UV 254/365 nm Visualiser

The ideal equipment for observing thin-layer chromatography (TLC) plates under UV light at 254 and 366 nm in a darkroom. Advantages

Viewing window with a protective filter.
If required, one of the lamps can be replaced with a white light lamp.
Non-fluorescent compounds are blocked by a selective filter.
Side window for working with thin-layer chromatography plates and preparative plates, even with aluminium-coated lamps.
Option to fit a chamber

Features

Illuminator made from polyacrylate glass.
Detection area: 200 x 200 mm
Lamp power: 8 x 6 W
Viewing window dimensions (W x H): 140 x 60 mm

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