Fluorescence in situ hybridisation is one of the fundamental methods of cytogenetic diagnosis, widely used in the fields of reproductive medicine and oncology.
FISH is an indispensable tool for the diagnosis of malignant tumours, aiding in diagnosis, predicting disease progression, determining the molecular subtype of a tumour, and selecting targeted therapy. In reproductive medicine, the method is used for pre-implantation analysis of embryos to detect possible chromosomal mutations, as well as in the prenatal and postnatal diagnosis of genetic disorders. As the gold standard for diagnosis, this method allows for the simultaneous analysis of a large number of genetic markers. The versatility of the matrix under investigation makes FISH a versatile, rapid and reliable diagnostic method.